Am J Cardiovasc Dis 2011;1(1):xx-xx

Original Article
Control of eotaxin-1 expression and release by resveratrol and its
metabolites in culture human pulmonary artery endothelial cells

Ching Jen Yang, Chia Yi Lin, Tze-chen Hsieh, Susan C. Olson, Joseph M. Wu

Department of Biochemistry & Molecular Biology, New York Medical College, Valhalla, New York 10595, USA.

Received March 23, 2011; April 19, 2011; Epub April 26, 2011; published June 1, 2011

Abstract: Population studies suggest that moderate red wine intake correlates with reduced risk of cardiovascular disease
(CVD); cardioprotection may attribute to consumption of red wine polyphenol resveratrol. Since inflammation plays a key
role in CVD, we investigated modulation of inflammation by resveratrol and its metabolites by determining the expression
and release of chemokine, eotaxin-1, in cultured human pulmonary artery endothelial cells (HPAEC) treated with
proinflammatory cytokines IL-13 and TNF-
α. Up-regulation of eotaxin-1 gene expression by IL-13 or TNF-α was confirmed
by RT-PCR, by reporter assays using eotaxin-1 gene promoter constructs, and by the changes in transcriptional factors
STAT6 and NF-κB. Exposure to resveratrol suppressed IL-13 and TNF-
α induced eotaxin-1 gene expression as well as
attenuated the eotaxin-1 promoter activity, in coordination with inhibition of expression of JAK-1, reduction in
phosphorylated-STAT6 and decreased p65 subunit of NF-κB. In addition, quantitative determination of eotaxin-1 release
using enzyme-linked immunosorbent assay (ELISA) showed increased eotaxin-1 release in response to treatment by IL-
13 and TNF-
α, which was effectively inhibited by resveratrol. Whether resveratrol metabolites affected eotaxin-1 was also
tested; piceatannol showed potency similar to resveratrol. We propose that control of eotaxin-1 expression and release by
proinflammatory cytokines in HPAEC may be considered as an in vitro model for screening and discovering polyphenols
with anti-inflammatory activities and cardioprotective potentials.(AJCD1103002).

Keywords: Resveratrol, piceatannol, eotaxin-1, STAT6, NF-κB

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Address all correspondence to:
Dr. Joseph M. Wu
Department of Biochemistry & Molecular Biology
New York Medical College
Valhalla, New York 10595,
Tel: 914-594-4891
Fax: 914-594-4058
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